M30 CytoDEATH™ |
(Prod. No. 10700/10750/ 10800/10830) |
BackgroundApoptosis proceeds through cleavage of various intracellular proteins. The proteases which mediate this process are referred to as caspases. One important caspase substrate is the intermediate filament protein keratin 18 (K18, Cytokeratin 18 or CK18) (Caulin et al., 1997), expressed in cells of simple epithelia.  |
The antibody M30 CytoDEATH™ recognizes a neo-epitope formed after caspase cleavage of keratin 18 at Asp396 (K18Asp396-NE, M30 neo-epitope) (Leers et al., 1999). M30 CytoDEATH™ does not bind native, unmodified K18 of normal cells. Therefore, M30 CytoDEATH™ is a very reliable and convenient tool for demonstration of apoptosis in single cells and tissue sections of epithelial origin.
The M30 CytoDEATH™ antibody is suitable for immunohistochemistry of tissue sections (formalin fixed and paraffin embedded or cryostat sections), immunocytochemistry and flow cytometry. Positive staining with M30 CytoDEATH™ will be confined to the cytoplasm of apoptotic cells.
Figure - The M30 CytoDEATH™ antibody is a unique and versatile tool for easy and reliable determination of apoptosis of suspension cells (ICC and FACS) and in tissue sections (IHC). Detection of apoptosis in a formalin-fixed and paraffin-embedded tissue section from a human colon cancer sample showing confined cytoplasmic staining for K18-NE using M30 CytoDEATH™ from PEVIVA. Secondary detection with anti-mouse IgG-biotin, streptavidin-POD and AEC as substrate counterstained with hematoxylin.
