Comparison between M65 EpiDeath® ELISA and M65® ELISA
The M65 EpiDeath® ELISA is intended for the same applications as the M65® ELISA.
The M65 EpiDeath® ELISA is based on the same monoclonal antibodies as the M65® ELISA. To achieve full compatibility with M30 Apoptosense® ELISA, mAb M5 is used as capture antibody (mAb M6 is used as capture antibody in the M65® ELISA).
Similarities and differences between M65 EpiDeath® and M65® ELISA:
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M65 EpiDeath® ELISA offers an even better discrimination between healthy individuals and patients suffering from diseases that lead to increases in circulating keratin 18 (K18). This is due to an improvement of binding specificity and lower signals in healthy controls.
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M65 EpiDeath® ELISA and M65® ELISA generate similar signals for patient samples that contain circulating K18 (r² = 0,84 in the material shown in the figure). The main difference between the tests is that most samples that show values in the range 200 – 300 U/L in the M65® ELISA will show values of < 100 U/L in the M65 EpiDeath® ELISA.
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Since M65 EpiDeath® and M65® ELISAs will not generate identical values; a particular experiment/project should use only one of these kits.
Determining cell death mode using the M65 EpiDeath® and M30 Apoptosense® ELISA
M65 EpiDeath® ELISA uses the same capture antibody as the M30 Apoptosense® ELISA, and “M30:M65 ratios” (see Kramer et al., 2004) measured using the M30 Apoptosense® and M65 EpiDeath® ELISA are expected to reflect cell death modes. In some instances, samples will generate higher signals in the M30 Apoptosense® ELISA test than in the M65 EpiDeath® ELISA test, probably reflecting how epitopes are presented in the complexes.
